论文标题
水中军团菌的声学和集体流体动力焦点的影响
Acoustophoresis of Legionella species in water and the influence of collective hydrodynamic focusing
论文作者
论文摘要
军团菌是革兰氏阴性，辅助细胞内和致病细菌，由于对水安装的广泛预防加热而造成人类健康的风险，并造成严重的能量损失。我们研究了几种军团菌，大肠杆菌，铜绿假单胞菌和Acanthamoeba castellanii，这是几种军团菌种类的声音驱动的运动 - 声学疫情。所有研究的细胞都可以在水中的超声驻波中进行声学操纵，因为它们具有非零的声学对比度，对所有细胞均为阳性，从而导致聚焦于驻波的压力节点。多体模拟表明，细胞浓度的增加可以显着加速由于流体动力相互作用引起的聚焦率 - 我们称之为集体流体动力焦点（CHF）的现象。结果构成了对细菌的声学操纵的基础，并可能铺平了通向水中军团菌的声学辅助检测的道路。
Legionella are gram-negative, facultative intracellular, and pathogenic bacteria that pose a risk for human health and cause significant energy losses due to extensive preventive heating-up of water installations. We investigate acoustically-driven motion - acoustophoresis - of several Legionella species, Escherichia coli, Pseudomonas aeruginosa, and Acanthamoeba castellanii, a common Legionella host in water. All the investigated cells can be acoustically manipulated in an ultrasonic standing wave in water, as they possess a non-zero acoustic contrast that is positive for all of the cells, leading to the focusing into pressure nodes of the standing wave. Multi-body simulations indicate that an increase in cell concentration could significantly accelerate the rate of focusing due to hydrodynamic interactions - a phenomenon that we call collective hydrodynamic focusing (CHF). The results form a foundation for acoustic manipulation of bacteria and could pave a path towards acoustically-aided detection of Legionella in water.
