论文标题
通过新兴的单分子和质谱法对蛋白质组进行取样
Sampling the proteome by emerging single-molecule and mass-spectrometry methods
论文作者
论文摘要
哺乳动物细胞的蛋白质分子比mRNA分子多约30,000倍。大量的分子和相关的较大动态范围对蛋白质组学技术的发展具有重大影响。我们研究了液相色谱串联质谱法（LC-MS/MS）和单分子计数的这些含义，并提供了有关通过LC-MS/MS在蛋白质组学实验中常规测量多少分子的估计值。我们审查了有助于计算LC-MS/MS数十亿蛋白质分子的策略，并建议这些策略可以使单分子方法受益，尤其是在减轻蛋白质组广泛动态范围的挑战方面。我们还研究了扩展单分子和质谱蛋白质组学方法的理论可能性，以量化构成我们细胞蛋白质组的数十亿个蛋白质分子。
Mammalian cells have about 30,000-fold more protein molecules than mRNA molecules. This larger number of molecules and the associated larger dynamic range have major implications in the development of proteomics technologies. We examine these implications for both liquid chromatography-tandem mass spectrometry (LC-MS/MS) and single-molecule counting and provide estimates on how many molecules are routinely measured in proteomics experiments by LC-MS/MS. We review strategies that have been helpful for counting billions of protein molecules by LC-MS/MS and suggest that these strategies can benefit single-molecule methods, especially in mitigating the challenges of the wide dynamic range of the proteome. We also examine the theoretical possibilities for scaling up single-molecule and mass spectrometry proteomics approaches to quantifying the billions of protein molecules that make up the proteomes of our cells.
