论文标题
如何制作和捕获用微型插曲制作伪用
How to make and trap a pseudo-vesicle with a micropipette
论文作者
论文摘要
我们提出了一种简单的方法，可以产生巨型脂质伪用叶子（顶部有油性盖的囊泡），被困在琼脂糖凝胶中。该方法只能使用常规的微型移动物来实现，并依靠液体琼脂糖中的水/油/水的形成。我们通过荧光成像来表征产生的囊泡，并通过成功插入α-蛋白酶跨膜蛋白来确定脂质双层的存在和完整性。最后，我们表明，可以通过缩进凝胶的表面来轻松地将囊泡机械地变形。
We present a simple method to produce giant lipid pseudo-vesicles (vesicles with an oily cap on the top), trapped in an agarose gel. The method can be implemented using only a regular micropipette and relies on the formation of a water/oil/water double droplet in liquid agarose. We characterize the produced vesicle with fluorescence imaging and establish the presence and integrity of the lipid bilayer by the successful insertion of α-Hemolysin transmembrane proteins. Finally, we show that the vesicle can be easily mechanically deformed, non-intrusively, by indenting the surface of the gel.
