论文标题
开放液滴微流体用于测试细菌中多药耐药性和抗生素弹性
Open Droplet Microfluidics for Testing Multi-Drug Resistance and Antibiotic Resilience in Bacteria
论文作者
论文摘要
正在研究现有抗生素的新组合以对抗细菌弹性。这需要具有合理成本，准确性，解决方案和吞吐量的检测技术。在这里，我们通过将液滴微流体，搜索算法和成像与广泛的视野相结合，为细菌培养物提供一个多药筛选平台。我们会远程改变细胞周围的化学微环境，并测试12种抗性细胞类型和化学物质的组合。荧光强度读数使我们能够在8小时内推断出对特定抗生素的细菌耐药性。该平台具有检测和鉴定细胞培养物，生物膜和微生物聚集体中细菌弹性的参数。
New combinations of existing antibiotics are being investigated to combat bacterial resilience. This requires detection technologies with reasonable cost, accuracy, resolution, and throughput. Here, we present a multi -drug screening platform for bacterial cultures by combining droplet microfluidics, search algorithms, and imaging with a wide field of view. We remotely alter the chemical microenvironment around cells and test 12 combinations of resistant cell types and chemicals. Fluorescence intensity readouts allow us to infer bacterial resistance to specific antibiotics within 8 hours. The platform has potential to detect and identify parameters of bacterial resilience in cell cultures, biofilms, and microbial aggregates.
