论文标题
通过狭窄而弯曲的通道，用于无标签成像的傅立叶全息内窥镜检查
Fourier holographic endoscopy for label-free imaging through a narrow and curved passage
论文作者
论文摘要
超薄无透镜纤维内窥镜提供了微创的研究，但由于需要事先校准纤维探针，因此它们主要是刚性类型。此外，大多数实现在荧光模式下工作，而不是无标签的成像模式，使其不适合医学和行业。在本文中，我们报告了具有0.87-μm空间分辨率的未染色组织的3D全息图像的完全柔韧性超薄纤维内窥镜。使用直径为200μm的裸露纤维束，我们设计了无透镜傅立叶全息成像构型，以选择性地检测来自生物组织的弱反射，这是无染色反射成像的关键步骤。开发了一种独特的算法，用于无校准的全息图像重建，使我们能够通过狭窄而弯曲的段落形象形象，无论纤维弯曲如何。我们证明了对常规内窥镜完全看不见的未染色大鼠肠组织的内窥镜反射成像。所提出的内窥镜将比以前的并发症最少更准确，更早的诊断。
Ultrathin lensless fibre endoscopes offer minimally invasive investigation, but they mostly operate as a rigid type due to the need for prior calibration of a fibre probe. Furthermore, most implementations work in fluorescence mode rather than label-free imaging mode, making them unsuitable for medicine and industry. Herein, we report a fully flexible ultrathin fibre endoscope taking 3D holographic images of unstained tissues with 0.87-μm spatial resolution. Using a bare fibre bundle as thin as 200-μm diameter, we design a lensless Fourier holographic imaging configuration to selectively detect weak reflections from biological tissues, a critical step for stain-free reflectance imaging. A unique algorithm is developed for calibration-free holographic image reconstruction, allowing us to image through a narrow and curved passage regardless of fibre bending. We demonstrate endoscopic reflectance imaging of unstained rat intestine tissues that are completely invisible to conventional endoscopes. The proposed endoscope will expedite more accurate and earlier diagnosis than before with minimal complications.
