论文标题
部分可观测时空混沌系统的无模型预测
Single-objective selective-volume illumination microscopy enables high-contrast light-field imaging
论文作者
论文摘要
通过选择性地通过第二个物镜镜头来选择性照明样品的相关子量，可以改善光场显微镜的性能[1-3]。在这里，我们使用倾斜的单光子照明路径或两光片照明来实现选择性体积激发，将这种方法推向单瞄准几何形状。 The elimination of the second orthogonally oriented objective to selectively excite the volume of interest simplifies specimen mounting;然而，这种单瞄准方法仍然降低了无体积背景，从而改善了图像对比度，有效分辨率和体积重建质量。我们通过成像实时发展斑马鱼来验证我们的新方法，以表明该技术可以同步捕获大型数据的成像数据，同时与标准显微镜样品安装兼容。
The performance of light-field microscopy is improved by selectively illuminating the relevant subvolume of the specimen with a second objective lens [1-3]. Here we advance this approach to a single-objective geometry, using an oblique one-photon illumination path or two-photon illumination to accomplish selective-volume excitation. The elimination of the second orthogonally oriented objective to selectively excite the volume of interest simplifies specimen mounting; yet, this single-objective approach still reduces out-of-volume background, resulting in improvements in image contrast, effective resolution, and volume reconstruction quality. We validate our new approach through imaging live developing zebrafish, demonstrating the technology's ability to capture imaging data from large volumes synchronously with high contrast, while remaining compatible with standard microscope sample mounting.
