论文标题
单分子相互作用时间的定量光谱
Quantitative spectroscopy of single molecule interaction times
论文作者
论文摘要
单分子荧光跟踪在NM尺度和MS-stormal分辨率上提供有关在生物环境中单个分子的动力学和相互作用的信息。虽然可以很好地表征分离的分子的动态行为，但当出现两个无法区分的分子之间的相互作用时，定量洞察力受到更大的限制。我们在这里通过为相互作用时间的光谱（即通过成像数据的相互作用常数推断）建立固体理论基础来解决这一方面。在这里突出显示了功率定律与相互作用时间分布的指数行为之间的非微不足道的交叉，以及指数项对微观反应速率（亲和力）的乘积的依赖性。我们的方法在模拟和实验数据集上得到了验证。
Single molecule fluorescence tracking provides information at nm-scale and ms-temporal resolution about the dynamics and interaction of individual molecules in a biological environment. While the dynamic behavior of isolated molecules can be characterized well, the quantitative insight is more limited when interactions between two indistinguishable molecules occur. We address here this aspect by developing a solid theoretical foundation for a spectroscopy of interaction times, i.e. the inference of interaction constants from imaging data. The non trivial crossover between a power law to an exponential behavior of the distribution of the interaction times is highlighted here, together with the dependence of the exponential term upon the product of the microscopic reaction rates (affinity). Our approach is validated on simulated as well as experimental datasets.
