论文标题
两相激光器的协同作用，用于生产适用于眼科的银纳米颗粒胶体胶体
Synergistic effect in two-phase laser procedure for production of silver nanoparticles colloids applicable in ophthalmology
论文作者
论文摘要
这项工作报告了基于眼科治疗方法的两相脉冲激光程序，在水溶液中生产Ag纳米颗粒（AGNP）。在这种情况下，AGNP应少于10 nm，并且尺寸分布狭窄。该尺寸尺度的纳米颗粒能够穿透复杂的眼屏屏障，从而确保有效的非侵入性药物向视网膜输送。在第一阶段，通过辐射，通过在水下的Ag靶点烧蚀Ag，大于20 nm，其基本波长为1064 nm，由ND：YAG激光产生。在第二阶段，为了减少拟声纳米颗粒的平均尺寸并正确调整尺寸分布，从同一激光源中，紫外线谐波（355 nm和266 nm）也会辐射水胶体。研究了关键激光参数的影响 - 波长，通量和激光暴露时间 - 研究了纳米颗粒形态的影响。最初胶体的最合适后处理是通过与第三（355 nm）和基本激光波长的第三（355 nm）和第四（266 nm）谐波的连续照射获得的。通过使用这种方法，诱导了AgNP的两种吸收光吸收机制之间的协同作用。结果，制造了无污染物的AGNP的无污染物胶体，尺寸低于10 nm，尺寸分布非常窄，标准偏差为1.6 nm。探索了AS生产的AGNP对革兰氏阳性和革兰氏阴性细菌和白色念珠菌的毒性作用。针对铜绿假单胞菌和大肠杆菌采取了最有效的作用。提出了与抗菌作用一起使用抗菌作用作为眼感染预防和治疗的非侵入性方法的潜在应用。
This work reports on the production of Ag nanoparticles (AgNPs) in water solution based upon two-phase pulsed laser procedure for ophthalmological therapeutic approaches. In this case, the AgNPs should be less then 10 nm and have a narrow size distribution. Nanoparticles of this sized-scale are capable to penetrate the complex ocular barriers, ensuring effective non-invasive drug delivery to retina. In the first phase, AgNPs larger than 20 nm were fabricated via laser ablation of a Ag target under water by irradiation with a fundamental wavelength of 1064 nm generated by a Nd:YAG laser. During the second phase, to reduce the mean size of the as-obtained nanoparticles and properly adjust the size distribution, the water colloids were additionally irradiated by ultraviolet harmonics (355 nm and 266 nm) from the same laser source. The effect of the key laser parameters - wavelength, fluence and laser exposure time - upon the nanoparticles morphology was studied. The most suitable post-ablation treatment of initial colloids was obtained by consecutive irradiation with the third (355 nm) and the fourth (266 nm) harmonics of the fundamental laser wavelength. By using this approach synergistic effect between two mechanisms of light absorption by AgNPs was induced. As a result contaminant-free colloids of AgNPs with a size inferior to 10 nm and a quite narrow size distribution with a standard deviation of 1.6 nm were fabricated. The toxic effect of the as-produced AgNPs on Gram-positive and Gram-negative bacteria and Candida albicans was explored. The most efficient action was reached against Pseudomonas aeruginosa and Escherichia coli. Potential application of the synthesized AgNPs colloidal aqueous solutions with antimicrobial action as a non-invasive method for ocular infections prevention and treatment was proposed.
