论文标题
纳米涂层细胞的预先定向的界面设计，以定向冷冻和解冻
Premelting-directed interfacial design of nanoshell-coated cells in directional freezing and thawing
论文作者
论文摘要
与软单元相互作用的冻结界面是冷冻保存中的核心问题。但是，大多数现有的冷冻保存方法都面临着诸如复杂处理和可控性不佳的挑战。本文中，我们报告了一种基于纳米技术的新方法，可以通过预言理论调节冰与细胞之间的界面相互作用。通过界面设计，可以通过控制细胞表面上的生物相容性纳米壳，可以改变细胞和冰之间的有效障碍物常数。细胞和冰之间的细胞膜的厚度进一步调节，涂层细胞的定向迁移发生在热梯度中，以实现涂层细胞的定向解冻。冷冻涂层细胞的死亡率降低表明，冰冻和解冻中的预言调节可以有效地保护细胞在冻结过程中冰的机械损伤，并在解冻过程中冰结晶。
Freezing interface interacting with soft cells is a core issue in cryopreservation. However, most of existing cryopreservation approaches face challenges such as complex processing and poor controllability. Herein we report a new, nanotechnology-based method to modulate interfacial interactions between ice and cells via the premelting theory. Through the interfacial design by controllably modifying biocompatible nanoshells on the surface of cells, the effective Hamaker constant between cells and ice can be modified. The thickness of premelted films between cells and ice is further regulated, and directional migration of coated cells occurs in a thermal gradient to achieve directional thawing of coated cells. The decreased mortality of freezing coated cells suggests the premelting modulation in freezing and thawing can effectively protect cells from mechanical damage of ice formation during freezing and ice recrystallization during thawing.
