论文标题
如何设计细胞介导的自组装胶体支架
How to design cell-mediated self-assembled colloidal scaffolds
论文作者
论文摘要
组织工程的关键步骤是3D生物相容性矩阵（支架）的设计和合成，以支持和指导细胞和组织生长的扩散。大多数现有的技术都依赖于在受控条件下的脚手架的处理，然后将其植入\ textit {in Vivo}，以及与生物相容性和植入过程有关的问题，这些问题仍然具有挑战性。作为替代方案，提议通过通过细胞介导的胶体颗粒的自组织来组装支架\ textit {in loco}。在这项研究中，我们将实验，基于粒子的模拟和平均场计算结合在一起，以表明通常，自组装支架尺度的大小与细胞与颗粒比。但是，我们发现该比率的最佳值，当细胞细胞粘附被抑制时，支架的大小是最大的。这些结果表明，可以通过调整胶体悬浮液中细胞之间的粘附来设计自组装支架的大小和结构。
A critical step in tissue engineering is the design and synthesis of 3D biocompatible matrices (scaffolds) to support and guide the proliferation of cells and tissue growth. Most existing techniques rely on the processing of scaffolds under controlled conditions and then implanting them \textit{in vivo}, with questions related to biocompatibility and the implantation process that are still challenging. As an alternative, it was proposed to assemble the scaffolds \textit{in loco} through the self-organization of colloidal particles mediated by cells. In this study, we combine experiments, particle-based simulations, and mean-field calculations to show that, in general, the size of the self-assembled scaffold scales with the cell-to-particle ratio. However, we found an optimal value of this ratio, for which the size of the scaffold is maximal when cell-cell adhesion is suppressed. These results suggest that the size and structure of the self-assembled scaffolds may be designed by tuning the adhesion between cells in the colloidal suspension.
