论文标题
连贯的傅立叶散射法揭示了大脑中的神经纤维交叉
Coherent Fourier scatterometry reveals nerve fiber crossings in the brain
论文作者
论文摘要
Menzel等人先前的模拟研究。 [物理。修订版X 10，021002（2020）]表明，通过人造神经纤维星座传播的光的散射模式包含有关组织子结构的有价值信息，例如具有交叉纤维的区域中的单个纤维方向。在这里，我们提出了一种使用通常具有入射光的连贯的傅立叶散射法来测量猴子和人脑组织中这些散射模式的方法。通过通过未染色的组织学脑切片传输非关注激光束（633 nm的波长），我们测量了小组织区域的散射模式（直径为0.1-1 mm），并表明它们与模拟的散射模式相符。我们揭示了多达三个交叉神经纤维束的单个纤维方向，交叉角度降至25°。
Previous simulation studies by Menzel et al. [Phys. Rev. X 10, 021002 (2020)] have shown that scattering patterns of light transmitted through artificial nerve fiber constellations contain valuable information about the tissue substructure such as the individual fiber orientations in regions with crossing nerve fibers. Here, we present a method that measures these scattering patterns in monkey and human brain tissue using coherent Fourier scatterometry with normally incident light. By transmitting a non-focused laser beam (wavelength of 633 nm) through unstained histological brain sections, we measure the scattering patterns for small tissue regions (with diameters of 0.1-1 mm), and show that they are in accordance with the simulated scattering patterns. We reveal the individual fiber orientations for up to three crossing nerve fiber bundles, with crossing angles down to 25°.
