论文标题
扫描显微镜OCT中的动态对比度
Dynamic contrast in scanning microscopic OCT
论文作者
论文摘要
虽然光学相干断层扫描（OCT）提供了低至1千分尺的分辨率，但由于缺乏散射对比度，很难可视化细胞结构。通过评估信号波动，使用时域全场OCT（FF-OCT）证明了显着的对比度增强，这使得细胞和亚细胞结构可见。动态OCT信号的推定原因是在亚微米范围内细胞结构的ATP依赖性运动，该运动提供了组织学的对比度。在这里，我们证明了与扫描频域OCT（FD-OCT）的动态对比。鉴于固有的截面成像几何形状，扫描FD-OCT提供了跨组织层的深度分辨图像，这是从组织病理学中知道的视角，比FF-OCT更快，更有效。较短的采集时间和层析成像深度剖面都降低了动态对比度对散装组织运动伪影的敏感性，并简化了它们在后处理中的校正。动态对比的实施使微观FD-OCT成为无染色组织组织学分析的有前途的工具。
While optical coherence tomography (OCT) provides a resolution down to 1 micrometer it has difficulties to visualize cellular structures due to a lack of scattering contrast. By evaluating signal fluctuations, a significant contrast enhancement was demonstrated using time-domain full-field OCT (FF-OCT), which makes cellular and subcellular structures visible. The putative cause of the dynamic OCT signal is ATP-dependent motion of cellular structures in a sub-micrometer range, which provides histology-like contrast. Here we demonstrate dynamic contrast with a scanning frequency-domain OCT (FD-OCT). Given the inherent sectional imaging geometry, scanning FD-OCT provides depth-resolved images across tissue layers, a perspective known from histopathology, much faster and more efficiently than FF-OCT. Both, shorter acquisition times and tomographic depth-sectioning reduce the sensitivity of dynamic contrast for bulk tissue motion artifacts and simplify their correction in post-processing. The implementation of dynamic contrast makes microscopic FD-OCT a promising tool for histological analysis of unstained tissues.
